Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
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Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye objects that are not within the resolution range of the normal eye. This process may be carried out by wide-field irradiation of the sample for example standard light microscopy and transmission electron microscopy or by scanning a fine beam over the sample for example confocal laser scanning microscopy and scanning electron microscopy.
Confocal microscopy , most frequently confocal laser scanning microscopy CLSM or laser confocal scanning microscopy LCSM , is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. This technique is used extensively in the scientific and industrial communities and typical applications are in life sciences , semiconductor inspection and materials science.
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